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. 2020 Feb 10;16(7):1238–1251. doi: 10.7150/ijbs.39768

Figure 3.

Figure 3

Axl-148b conjugate inhibits formation and growth of mammospheres derived from AXL+ but not AXL- tumor cells. (A) Experimental scheme related to mammosphere assays referring to 4175-TGL or SKBR3 breast cancer cell lines. Cells were plated and grown in suspension for 5 days and treated with 200/400 nmol/L of axl, axl-148b or scr-148b aptamers at day 0, 3, 5, as indicated (numbers with squares). (B-C) Control (pLenti-empty + pLenti4/V5-empty) or miR-148b-overexpressing (pLenti4/V5-148b) 4175-TGL breast cancer cells were put in culture (day 0, suspension) and formation and size of spheres evaluated at day 5. (D-G) Formation and growth of spheres derived from 4175-TGL or SKBR3 breast cancer cells at day 5, treated as in (A). (B, D, F) Box-and-whisker plots for number of 4175-TGL or SKBR3 derived-mammospheres in 50 µl of culture volume at day 5 shown as mean±SEM. (C, E, G) Top: representative images of 4175-TGL or SKBR3 derived-mammospheres at day 5. Bottom: box-and-whisker plots of sphere size, shown as mean±SEM of sphere length (µm); black lines correspond to measurements for size. (B-G) 2 or 3 independent experiments (with triplicates) were performed and representative results are shown. scr= scramble; ns = not significant; * p < 0.05, ** p < 0.01, *** p < 0.001; SEM = Standard Error of Mean; scale bar = 25 µm (C, E, G).