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. 2020 Feb 10;16(7):1218–1229. doi: 10.7150/ijbs.40338

Figure 4.

Figure 4

Overexpression of PMP22 ablates the inhibitory effects of miR-139-5p in GC cells. (A) The q-RT-PCR results show the expression of PMP22 in the SGC7901 and BGC823 cells infected with PLV-PMP22 plasmids. (B) SGC7901 and BGC823 cells were infected with PLV-PMP22 plasmids, and the cells were harvested for western blot analysis with antibodies against PMP22 and Actin. (C) Cell proliferation was determined by CCK-8 assay. The ectopic expression of PMP22 rescued the miR-139-5p-mediated inhibition of cell proliferation in SGC7901 and BGC cells. (D) Statistical analysis of the CCK8 assay results at 72 h in C. (E) The ectopic expression of PMP22 rescued the miR-139-5p-mediated inhibition of cell proliferation in SGC7901 and BGC cells. (F) Statistical analysis of E. *p < 0.05, and **p < 0.01, ***p < 0.001.