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. 2020 Feb 10;16(7):1218–1229. doi: 10.7150/ijbs.40338

Figure 5.

Figure 5

MiR-139-5p represses PMP22 by inhibting the NF-κB pathway. (A) Luciferase reporter assays of NF-κB. SGC7901 cells were transfected with miR-139-5p. After 48h, cells were treated with or without TNFα, and then luciferase reporter assays were performed. (B) Luciferase reporter assays of NF-κB. Ectopic expression of PMP22 rescued the miR-139-5p-mediated inhibition of NF-κB reporter gene activity in SGC7901 cells. (C) The q-RT-PCR analysis of the expression of PMP22 in SGC7901 cells infected with PLV-shPMP22 plasmids. (D) SGC7901 cells were infected with PLV-shPMP22 plasmids, and the cells were harvested for western blot analysis with antibodies against PMP22 and Actin. (E) NF-κB reporter gene activity was detected in the SGC7901-shPMP22 cell line after TNFα stimulation. (F) Luciferase reporter assays. PMP22 knockdown increased the inhibitory effects induced by miR-139-5p on NF-κB reporter gene activity in SGC7901 cells.