Figure 2.

Wn5a is essential for TAMs to promote CRC cells proliferation, migration and macrophage recruitment. A RT-qPCR analysis of Wnt5a mRNA expression in Wnt5a⁺ TAM transfected with sh-NC or sh-Wnt5a. Error bars, SEM. B Western blot analysis of Wnt5a protein expression in Wnt5a⁺ TAM transfected with sh-NC or sh-Wnt5a. C Cell viability of CRC cells cultured with fresh medium (control), supernatant from M0 macrophages, sh-NC or sh-Wnt5a TAMs. Error bars, SEM. D Transwell migration assay of CRC cells treated with conditioned medium. (magnification, ×100). E Quantification analysis of migratory cells in five fields was counted manually. Error bars, SEM. F Chemotaxis analysis of THP-1 macrophages toward fresh medium (control), supernatant from M0 macrophages, sh-NC or sh-Wnt5a TAMs. (magnification, ×100). G Quantification analysis of chemotactic cells in five fields was counted manually. Error bars, SEM. *P<0.05. **P< 0.01. ***P<0.001