Skip to main content
. 2020 Feb 4;16(6):1023–1034. doi: 10.7150/ijbs.40535

Figure 4.

Figure 4

Wnt5a regulates CCL2 expression through CaMKII-ERK1/2 pathway. A Western blot analysis of p-ERK1/2 level at the indicated time in M0 macrophages treated with Wnt5a. B Immunofluorescence analysis for ERK1/2 in M0 macrophages treated with Wnt5a. Bar = 25μm. C ELISA analysis of CCL2 expression in Wnt5a-treated M0 macrophages with or without U0126. Error bars, SEM. D Western blot analysis of Wnt5a-treated M0 macrophages with or without CK59 or U0126. E ELISA analysis of CCL2 expression in Wnt5a-treated M0 macrophages with or without CK59. Error bars, SEM. F Western blot analysis of TAMs transfected with sh-Wnt5a or sh-NC. G Western blot analysis of TAMs with or without Box5 or U0126 or CK59. H ELISA analysis of CCL2 secretion level in TAMs with or without Box5 or U0126 or CK59. Error bars, SEM. ***P<0.001