Figure 5.

Knockdown of the CPCM components caused oncogenic phenotype defects. (A and B) The knockdown of CPCM components decreased cell proliferation. The CPCM component knockdown cells in HT29 (A) and HCT-116 (B) backgrounds were subjected to determine cell proliferation using an MTT assay. ^**P <0.01. (C) The knockdown of CPCM components decreased colony formation. The same cells as used in (A and B) were seeded into six-well plates with a density of 10³ cells per well, followed by continuously growing for two weeks. Colonies were stained with 0.2% crystal violet. (D) The knockdown of CPCM components inhibited cell invasion. The same cells as used in (A and B) were seeded into the upper chamber of Boyden chambers and incubated in 37°C for 24 h. Cells in the lower chambers were fixed in methanol and stained with 0.2% crystal violet. Bars=50 μm.