Skip to main content
. 2020 Feb 4;16(6):1071–1085. doi: 10.7150/ijbs.41230

Figure 6.

Figure 6

Inhibition of CPCM components decreased their occupancies on the promoters of CUL4A/4B. (A and B) The knockdown of CPCM components decreased their occupancies on the promoters of CUL4A/4B. The HT29, c-Myc-KD, CARM1-KD and p300-KD cells were subjected to ChIP assays using anti-c-Myc, anti-CARM1, anti-p300 or IgG for immunoprecipitation. The purified DNA was used to examine the enrichment of CPCM components on the promoters of CUL4A (A) and CUL4B (B). **P < 0.01. (C and D) Inhibition of CPCM components impaired their enrichment on the promoters of CUL4A/4B. The HT29 cells were treated with 5 μM sAJM, 20 μM CARM-IN-1 or 50 nM C646 for 6 h, followed by treatment with 200 ng/mL IL-6 for another 6 h. The resulting cells were subjected to ChIP assays using anti-c-Myc, anti-CARM1, anti-p300 or IgG for immunoprecipitation. The purified DNA was used to examine the enrichment of CPCM components on the promoters of CUL4A (C) and CUL4B (D). **P < 0.01 and ***P < 0.001.