Figure 2.

Object training activates canonical Wnt/β-catenin-dependent signaling in the dorsal hippocampus of male mice. (A) Protein levels of Wnt7a were significantly increased relative to controls 5 minutes after training. (B) Phospho-GSK3β protein levels were significantly increased relative to controls 30 minutes after training. (C, D) At all time points following training, protein levels of β-catenin (C) and Cyclin D1 (D) were significantly increased relative to controls. Protein levels were normalized to β-actin. Each bar represents the mean ± SEM percent change from vehicle (*P ≤ 0.05 relative to controls). Insets show representative Western blots. Adapted with permission from Fortress and others (2013a).