Figure 6.

The effect of S100A9 on the phosphorylations of MAPKs and STAT3 in osteocytes. MLO-Y4-A2 cells were treated with S100A9 for 0.5–2 hours, and cell lysate from osteocytes was extracted. (a) Phosphorylations of MAPKs including p38, ERK, and JNK and STAT3 were determined by Western blotting. ((b)–(e)) Phosphorylation levels of MAPKs and STAT3 were analyzed by densitometric measurement. Data were normalized by nonphosphorylated total factors. Data are means ± SD (n = 3). N.S indicates no significant difference between the indicated groups and control group.