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. 2020 Feb 19;2020:5393041. doi: 10.1155/2020/5393041

Table 2.

Unsaturated lipid profile of MCF-7 and MDA-MB-231 cells after resveratrol treatment.

FA ID Lipid name R.T. MCF-7 MDA-MB-231
Control RESV Control RESV
(μg/μL) (μg/μL)
C16 : 1n9 7-Hexadecenoic acid 21.867 0.027 ± 0 0.040 ± 0.025 0.112a 0.095a
C16 : 1n7 Palmitoleic acid 22.104 0.223 ± 0.133 0.252 ± 0.061 0.093 ± 0.005 0.131 ± 0.030
C17 : 1n7 Cis-10-heptadecanoic acid 27.261 0.027 ± 0.014 0.026 ± 0.01 0.023 ± 0.005 0.038 ± 0.013
C18 : 2n6 Linoleic acid 32.871 0.036 ± 0.012 0.038 ± 0.002 0.038 ± 0.012 0.056 ± 0.001
C18 : 1n9c Oleic acid 33.820 0.542 ± 0.076 0.831 ± 0.478 0.460 ± 0.014 0.718 ± 0.206
C18 : 1n9t Elaidic acid 34.094 0.419 ± 0.168 0.334 ± 0.002 0.162 ± 0.005 0.234 ± 0.036
C20 : 4n6 Arachidonic acid 43.802 0.073 ± 0.014 0.102 ± 0.064 0.123 ± 0.071 0.235 ± 0.159
C20 : 5n3 Eicosapentaenoic acid (EPA) 44.181 0.011 ± 0.001 0.019 ± 0.016 0.040 ± 0.020 0.060 ± 0.036
C20 : 3n6 8, 11, 14-Eicosatrienoic acid 45.067 0.016 ± 0.001 0.025 ± 0.020 0.022 ± 0.008 0.053 ± 0.031
C20 : 2n6 11, 14-Eicosadienoic acid 47.228 0.005a 0.007 ± 0.004 0.007a 0.012 ± 0.003
C20 : 1n9 Cis-11-Eicosaenoic acid 48.739 0.038 ± 0.013 0.042 ± 0.030 0.013a 0.011 ± 0.007
C20 : 1 49.254 0.036 ± 0.025 0.027 ± 0.012 ND 0.002a
C22 : 6n3 4, 7, 10, 13, 16, 19-Docosahexaenoic acid (DHA) 57.155 0.052 ± 0.010 0.086 ± 0.057 0.084 ± 0.049 0.160 ± 0.108
C22 : 1n9 Erucic acid 61.184 0.014 ± 0.010 0.014 ± 0.004 0.010 ± 0.004 0.008 ± 0.004
C24 : 1n9 Nervonic acid 75.325 0.034 ± 0.019 0.038 ± 0.013 0.012 ± 0.006 0.019 ± 0.002
Total unsaturated lipid content 1.553 1.881 1.199 1.832

FA ID: fatty-acid identification; RESV: resveratrol; R.T.: retention time; ND: Not determined. aFatty acid detected in only one independent experiment. MCF-7 and MDA-MB-231 (1 × 107) cells after resveratrol (80 and 200 μM, respectively) or DMSO 0.1% (control) treatment for 24 h were subjected to lipid extraction. Lipid extracts were analyzed by GC/MS. Results are expressed as mean ± S.E.M., n = 2.