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. 2020 Feb 25;8:98. doi: 10.3389/fchem.2020.00098

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Copyright © 2020 Vasta, Feng, Tasumi, Abernathy, Bianchet, Wilson, Paschinger, Wang, Iqbal, Ghosh, Amin, Smith, Brown and Vista.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CvGals mediate P. marinus trophozoites adhesion onto oyster hemocytes. (A) Carbohydrate -specific binding of rCvGal1 to P. marinus trophozoites in the presence and absence of thiodigalactose (inhibits rCvGal1 binding) or glucose (does not inhibit rCvGal1 binding) was analyzed by immunofluorescence staining. Scale bar, 10 μm. (B) Adhesion of P. marinus trophozoites to hemocytes was enhanced by addition of recombinant CvGal1 or CvGal2 (left) or inhibited by addition of anti-CvGal1 or anti-CvGal2 antibody (right). *Indicates significant difference (p < 0.05) from control sample [Adapted from Tasumi and Vasta (2007), and Feng et al. (2013) with permission from the American Association of Immunologists and the American Society for Biochemistry and Molecular Biology].