Allo increased brain mitochondrial efficiency and biogenesis in OVX 3xTgAD mice. (A) Six weeks of OVX induced a decline in mitochondrial respiration in 3xTgAD mice, compared to the sham group. Allo and E2 treatment significantly reversed the decreased mitochondrial respiration in OVX 3xTgAD mice (p < 0.05, n = 6 per group). (B) OVX induced a significant rise in proton leak in 3xTgAD mice, which was significantly reversed by both Allo and E2 treatment by a comparable magnitude (p < 0.05, n = 6 per group). (C) Allo and E2 treatment significantly restored OVX-induced decrease in mtDNA/nDNA ratio to levels comparable to the sham group (p < 0.05, n = 3–4 per group)