Figure 5.

TEER in CFBE41o- monolayers following treatment with K279a CS. CFBE41o- cells were seeded at a density of 5 × 10⁵ cells/cm² onto clear permeable filter inserts (6.5 mm diameter, 0.4 μm pore size). Cells were grown for 7 days in MEM supplemented with 10% FCS and medium was changed on alternate days. Prior to treatment, cells were washed with DPBS to remove any residual FCS and the media replaced with serum free medium (SFM) and allowed to equilibrate for a further 48 h. The day before treatment cells were placed in fresh SFM overnight. CFBE41o- cell monolayers were left untreated (control) or treated with K279a CS (CS) (protease activity = 5 × 10³ RFU/min) in the absence or presence of PMSF (1 mM). Transepithelial electrical resistance (TEER) was measured using an EVOM epithelial voltmeter in an Endohm-6 chamber. To construct TEER curves, measurements were taken every 2 h. (A) TEER curve of apically treated cells. (B) TEER curve of basolaterally treated cells. All results are representative of three independent experiments. Results are expressed as relative TEER to time zero (T0). Treatment vs. control: ****p ≤ 0.0001, ***p ≤ 0.001; Two-way-ANOVA followed by Tukey post-hoc test for multiple comparisons.