Table 2. Multiplex antibody panel description.
Protein | Concentration | Species | Clone | Company | RRID_AB: |
---|---|---|---|---|---|
CD4 | 1 µg/ml | rabbit Mab | EPR6855 | Abcam | N/A |
HLA-DR | 1 µg/ml | mouse IgG2b | SPM288 | Abcam | 1125217 |
TAP2 | 1 µg/ml | mouse IgG1 | TAP2.17 | Abcam | N/A |
CD141 | 1 µg/ml | rabbit Mab | EPR4051 | Abcam | 2201805 |
MYC | 1 µg/ml | rabbit Mab | EP121 | Sigma Aldrich | N/A |
FOXP3 | 1 µg/ml | mouse IgG1 | 236A/E7 | Abcam | 445284 |
MX1 | 1 µg/ml | Rabbit | Abcam | 10678925 | |
LAG3 | 1 µg/ml | mouse IgG1 | 11E3 | Abcam | 776102 |
PD-L1 | 1 µg/ml | rabbit Mab | 28–8 | Abcam/Epitomics | 2687878 |
CD1a | 1 µg/ml | Rb mAb | EP3622 | Abcam/Epitomics | 626957 |
CD123 | 1 µg/ml | mouse IgG2b | NCL-L-CD123 | Leica-Microystem/Novocastra | 10555271 |
Phospho-Stat1 | 1 µg/ml | rabbit Mab | 58D6 | Cell Signaling | 561284 |
CD20 | 1 µg/ml | mouse IgG2a | L26 | Dako | 782024 |
CD1a | 1 µg/ml | mouse IgG1 | O10 | Dako | N/A |
CD1c | 1 µg/ml | mouse IgG1 | 2D4 | Dako | 2623049 |
PRDM1 | 1 µg/ml | rat | 6D3 | Dako | 628168 |
S100AB | 1 µg/ml | rabbit | Dako | N/A | |
CD56 | 1 µg/ml | mouse IgG1 | 123C3.D5 | Neomarkers | 627127 |
Ki-67 | 2 µg/ml | mouse IgG2a | UMAB107 | Origene | 2629145 |
Lysozyme | 1 µg/ml | rabbit | Origene | 1004766 | |
PD-1 | 1 µg/ml | mouse IgG2a | UMAB197 | Origene | 2629198 |
TIM3 | 1 µg/ml | goat | R and D | 355235 | |
CXCL13 | 1 µg/ml | mouse IgG1 | 53610 | R and D | 2086049 |
OX40 | 1 µg/ml | mouse IgG1 | Ber-ACT35 | Santa Cruz | 626897 |
IRF4 | 1 µg/ml | goat | M-17 | Santa Cruz | 2127145 |
cMAF | 1 µg/ml | rabbit | M-153 | Santa Cruz | 638562 |
BCL6 | 1 µg/ml | rabbit | N3 | SCBT | 1158074 |
CD16 | 1 µg/ml | mouse IgG2a | 2H7 | SCBT | 563508 |
CD68 | 1 µg/ml | mouse IgG3 | PGM1 | Thermo Fisher | 10979558 |
p16 | 1 µg/ml | mouse IgG2a | JC8 | SCBT | 785018 |
MelanA | 1 µg/ml | rabbit Mab | A19-P | NovusBio | 1987285 |
podoplanin | 1 µg/ml | rat IgG2a | NZ-1.2 | Sigma Aldrich | 10920577 |
CD69 | 1 µg/ml | rabbit | Sigma Aldrich | 2681157 | |
CD3 | 1 µg/ml | rabbit | Sigma Aldrich | 2335677 | |
GBP1 | 1 µg/ml | rat | 4D10 | Sigma Aldrich | 828964 |
Langerin | 1 µg/ml | rabbit | Sigma Aldrich | 1078453 | |
IRF8 | 1 µg/ml | rabbit | Sigma Aldrich | 1851904 | |
CD8 | 1 µg/ml | rabbit Mab | SP16 | Thermo Fisher | 627211 |
CD138 | 1 µg/ml | mouse IgG1 | MI-15 | Thermo Fisher | 10987019 |