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. 2020 Feb 14;9:e53008. doi: 10.7554/eLife.53008

Table 2. Multiplex antibody panel description.

Protein Concentration Species Clone Company RRID_AB:
CD4 1 µg/ml rabbit Mab EPR6855 Abcam N/A
HLA-DR 1 µg/ml mouse IgG2b SPM288 Abcam 1125217
TAP2 1 µg/ml mouse IgG1 TAP2.17 Abcam N/A
CD141 1 µg/ml rabbit Mab EPR4051 Abcam 2201805
MYC 1 µg/ml rabbit Mab EP121 Sigma Aldrich N/A
FOXP3 1 µg/ml mouse IgG1 236A/E7 Abcam 445284
MX1 1 µg/ml Rabbit Abcam 10678925
LAG3 1 µg/ml mouse IgG1 11E3 Abcam 776102
PD-L1 1 µg/ml rabbit Mab 28–8 Abcam/Epitomics 2687878
CD1a 1 µg/ml Rb mAb EP3622 Abcam/Epitomics 626957
CD123 1 µg/ml mouse IgG2b NCL-L-CD123 Leica-Microystem/Novocastra 10555271
Phospho-Stat1 1 µg/ml rabbit Mab 58D6 Cell Signaling 561284
CD20 1 µg/ml mouse IgG2a L26 Dako 782024
CD1a 1 µg/ml mouse IgG1 O10 Dako N/A
CD1c 1 µg/ml mouse IgG1 2D4 Dako 2623049
PRDM1 1 µg/ml rat 6D3 Dako 628168
S100AB 1 µg/ml rabbit Dako N/A
CD56 1 µg/ml mouse IgG1 123C3.D5 Neomarkers 627127
Ki-67 2 µg/ml mouse IgG2a UMAB107 Origene 2629145
Lysozyme 1 µg/ml rabbit Origene 1004766
PD-1 1 µg/ml mouse IgG2a UMAB197 Origene 2629198
TIM3 1 µg/ml goat R and D 355235
CXCL13 1 µg/ml mouse IgG1 53610 R and D 2086049
OX40 1 µg/ml mouse IgG1 Ber-ACT35 Santa Cruz 626897
IRF4 1 µg/ml goat M-17 Santa Cruz 2127145
cMAF 1 µg/ml rabbit M-153 Santa Cruz 638562
BCL6 1 µg/ml rabbit N3 SCBT 1158074
CD16 1 µg/ml mouse IgG2a 2H7 SCBT 563508
CD68 1 µg/ml mouse IgG3 PGM1 Thermo Fisher 10979558
p16 1 µg/ml mouse IgG2a JC8 SCBT 785018
MelanA 1 µg/ml rabbit Mab A19-P NovusBio 1987285
podoplanin 1 µg/ml rat IgG2a NZ-1.2 Sigma Aldrich 10920577
CD69 1 µg/ml rabbit Sigma Aldrich 2681157
CD3 1 µg/ml rabbit Sigma Aldrich 2335677
GBP1 1 µg/ml rat 4D10 Sigma Aldrich 828964
Langerin 1 µg/ml rabbit Sigma Aldrich 1078453
IRF8 1 µg/ml rabbit Sigma Aldrich 1851904
CD8 1 µg/ml rabbit Mab SP16 Thermo Fisher 627211
CD138 1 µg/ml mouse IgG1 MI-15 Thermo Fisher 10987019