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. 2020 Feb 25;14:36. doi: 10.3389/fncel.2020.00036

Figure 4.

Figure 4

Driving depolarizing GABAA transmission does not decrease glutamatergic synapse numbers. (A) Sample mIPSC traces with superimposed mean traces in color for Ctrl (n = 104) and DZP (n = 197) conditions (left), and enlarged overlay of mean Ctrl and DZP mIPSCs (right). DZP was applied at 5 μM. (B,C) Summary data for mIPSC decay constant (Ctrl 13.7 ± 1.73 ms, DZP 19.1 ± 1.15 ms, p = 0.007, n = 3 cells) and charge transfer (Ctrl 0.063 ± 0.015 pC; DZP 0.101 ± 0.013 pC, p = 0.006, n = 3 cells). (D) Time course of DZP treatment in organotypic slices. (E,F) Spine density after 3–5 DIV DZP treatment (Ctrl 0.321 ± 0.02, n = 116; DZP 0.36 ± 0.02, n = 88; N = 6; p = 0.11, Mann–Whitney). (G) Representative traces of mEPSCs following 3–5 DIV treatment with DZP. (H) mEPSC frequency summary plot (Ctrl 0.27 ± 0.02 Hz, n = 9; DZP 0.25 ± 0.04 Hz, n = 8; p = 0.41 Mann–Whitney). (I) mEPSC amplitude summary plot (Ctrl 18.3 ± 0.7 pA, n = 9; DZP 17.5 ± 0.6 pA, n = 8; p = 0.39). **p < 0.01.