TABLE 4.
Messenger RNA abundance (log-2 scale) in liver tissue from Holstein cows individually fed a control diet or the control plus rumen-protected Met (0.9 g/kg dry matter intake) during the last 28 d before parturition1
| Treatment | P value | ||||
|---|---|---|---|---|---|
| Item | Control | Met | Treat | Time | Treat × Time |
| TCA cycle, log-2 | |||||
| CS | 2.24 ± 0.08 | 2.11 ± 0.08 | 0.342 | 0.003 | 0.416 |
| ACO2 | 4.74 ± 0.10 | 4.92 ± 0.10 | 0.045 | 0.005 | 0.367 |
| IDH1 | 2.99 ± 0.07 | 3.07 ± 0.07 | 0.496 | 0.012 | 0.202 |
| OGDH | 1.13 ± 0.09 | 1.04 ± 0.09 | 0.124 | 0.222 | 0.433 |
| SUCLA2 | 2.99 ± 0.07 | 3.07 ± 0.07 | 0.325 | 0.006 | 0.205 |
| SDHA | 1.13 ± 0.13 | 1.14 ± 0.13 | 0.186 | 0.173 | 0.346 |
| FH | 1.89 ± 0.11 | 2.12 ± 0.11 | 0.041 | 0.007 | 0.164 |
| MDH1 | 0.89 ± 0.16 | 0.87 ± 0.16 | 0.267 | 0.030 | 0.371 |
| Met and folic acid cycle, log-2 | |||||
| BHMT | 1.99 ± 0.07 | 1.07 ± 0.07 | 0.493 | 0.004 | 0.201 |
| MTR | 1.13 ± 0.13 | 1.04 ± 0.13 | 0.125 | 0.724 | 0.550 |
| MAT1A | 1.28 ± 0.12 | 1.49 ± 0.12 | 0.064 | 0.063 | 0.488 |
| GNMT | 0.98 ± 0.15 | 1.01 ± 0.15 | 0.236 | 0.232 | 0.323 |
| AHCY | 1.14 ± 0.11 | 1.15 ± 0.11 | 0.423 | 0.214 | 0.412 |
| Transsulfuration pathway, log-2 | |||||
| CBS | 0.74 ± 0.07 | 0.73 ± 0.07 | 0.073 | 0.006 | 0.491 |
| CTH | 1.58 ± 0.05 | 1.64 ± 0.05 | 0.395 | 0.008 | 0.135 |
| CDO | 0.13 ± 0.09 | 0.12 ± 0.09 | 0.466 | 0.010 | 0.416 |
| GSR | 0.62 ± 0.06 | 0.66 ± 0.06 | 0.514 | 0.004 | 0.583 |
| GPX1 | 0.34 ± 0.07 | 0.35 ± 0.07 | 0.887 | 0.024 | 0.317 |
| Gluconeogenesis, log-2 | |||||
| PC | 2.11 ± 0.07 | 2.14 ± 0.07 | 0.425 | 0.003 | 0.453 |
| PCK1 | 2.09 ± 0.11 | 2.29 ± 0.11 | 0.093 | 0.001 | 0.218 |
Values are means ± pooled SEMs, n = 10 per treatment group. ACO2, aconitase 2; AHCY, adenosylhomocysteinase; BHMT, betaine-homocysteine S-methyltransferase; CBS, cystathionine β-synthase; CDO, cysteine dioxygenase; CS, citrate synthase; CTH, cystathionine γ-lyase; FH, fumarate hydratase; GNMT, glycine N-methyltransferase; GPX1, glutathione peroxidase 1; GSR, glutathione-disulfide reductase; IDH1, isocitrate dehydrogenase (NADP(+)) 1; MAT1A, methionine adenosyltransferase 1A; MDH1, malate dehydrogenase 1; MTR, 5-methyltetrahydrofolate-homocysteine methyltransferase; ND, not detected; OGDH, oxoglutarate dehydrogenase; PC, pyruvate carboxylase; PCK1, phosphoenolpyruvate carboxykinase 1; SDHA, succinate dehydrogenase complex flavoprotein subunit A; SUCLA2, succinate-CoA ligase ADP-forming β-subunit; TCA, tricarboxylic acid.