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. 2020 Jan 17;12(2):1417–1432. doi: 10.18632/aging.102691

Figure 6.

Figure 6

Cardiomyocyte mitochondrial metabolism is disrupted by SRV2-induced mitochondrial fission. (A) ATP production was measured in cardiomyocytes after transfection of siRNA against SRV2 and addition of FCCP to the culture medium. (BD) RNA was isolated from LPS-treated cardiomyocytes and qPCR was performed to analyze ETC-1/2/3 transcript levels after SRV2 siRNA transfection and FCCP treatment. (EG) Cardiomyocyte ETC-1/2/3 activities were measured via ELISA after SRV2 siRNA transfection and FCCP treatment. (HI) The JC-1 probe was used to evaluate mitochondrial membrane potential in cardiomyocytes after SRV2 siRNA transfection and FCCP treatment; red-to-green fluorescence ratios indicate alterations in mitochondrial membrane potential. *p<0.05 vs. control group, #p<0.05 vs. LPS group, @p<0.05 vs. LPS+si-SRV2 group.