| Gene |
Primer sequence (5’-3’) |
| U6 |
Forward:CGCTTCGGCAGCACATATAC |
|
Reverse:AAATATGGAACGCTTCACGA |
| miR-200a |
Forward:GCGCGTAACACTGTCTGGTAA |
|
Reverse:AGTGCAGGGTCCGAGGTATT |
| miR-200b |
Forward:GCGCATCTTACTGGGCAGC |
|
Reverse:AGTGCAGGGTCCGAGGTATT |
| miR-200c |
Forward:GCGCGTCTTACCCAGCAGT |
|
Reverse:AGTGCAGGGTCCGAGGTATT |
| GAPDH |
Forward:TGTGGGCATCAATGGATTTGG |
| (human) |
Reverse:ACACCATGTATTCCGGGTCAAT |
| GAPDH |
Forward:TTGTCATGGGAGTGAACGAGA |
| (mouse) |
Reverse:CAGGCAGTTGGTGGTACAGG |
| PD-L1 |
Forward:GCTGCACTAATTGTCTATTGGG |
| (human) |
Reverse:CACAGTAATTCGCTTGTAGTCG |
| PD-L1 |
Forward:AAGCCTCAGCACAGCAACTTCAG |
| (mouse) |
Reverse:TGTAGTCCGCACCACCGTAGC |
| PTEN |
Forward:ATGTTCAGTGGCGGAACTTG |
| (human) |
Reverse:CACACAGGTAACGGCTGAGG |
| PTEN |
Forward:TGGATTCGACTTAGACTTGACCT |
| (mouse) |
Reverse:GCGGTGTCATAATGTCTCTCAG |
