Figure 4.

H4K16 hyperacetylation in old and SIRT2-depleted oocytes. (A) Control and SIRT2 knockdown (siSirt2) oocytes were immunolabeled with H4K16ac antibody (green) and counterstained with propidium iodide (red) for DNA. Representative confocal sections are shown. (B) Quantification of fluorescence intensity of acetylated H4K16 in control and siSirt2 oocytes. (n=10 for each group). (C) Young, old and old+Mel oocytes were immunolabeled with H4K16ac antibody (green) and counterstained with propidium iodide (red) for DNA. Representative confocal sections are shown. (D) Quantification of fluorescence intensity of acetylated H4K16 in young, old and old+Mel oocytes (n=10 for each group). Data are expressed as the mean ± SD from three independent experiments. *P<0.05 vs. controls. Scale bars: 5 μm.