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. 2020 Jan 16;12(2):1114–1127. doi: 10.18632/aging.102648

Figure 6.

Figure 6

The overexpression of Romo1 promoted M2 polarization through mTORC1 signaling pathway in macrophages. (A) The expression of the indicated proteins was examined by western blotting in the control and Romo1-overexpressed macrophages with or without treatment of LPS (NT: without treatment; ST: LPS stimulation). (B) The ECAR and OCR of control and Romo1-overexpressed (with or without treatment of 60μM 3-BDO) macrophages were measured under basal conditions followed by the indicated treatment. (C) The control and Romo1-overexpressed (with or without treatment of 60μM 3-BDO) macrophages were respectively analyzed by flow cytometry with M1 or M2 markers. (D) The levels of 2-NBDG were analyzed by flow cytometry in control and Romo1-overexpressed (with or without treatment of 60μM 3-BDO) macrophages. (E) The oxygen uptake rates were measured and quantified in the control and Romo1-overexpressed (with or without treatment of 60μM 3-BDO) macrophages. (F) The production of IL-10, TGF-β, TNF-α or IL-6 was respectively analyzed by ELISA in control and Romo1-overexpressed (with or without treatment of 60μM 3-BDO) macrophages. Data are representative of at least three independent experiments and are presented as mean ± SD. ns, not significant; *, P < 0.05; **, P < 0.01.