Table 1. Characteristics of MIHR mutant colonies.

Colony name	Predominant mutations (minor mutations)#	Medusa sex	Polyp colony*	Medusa gonads
n5-23	−4 nt, +74 nt (−8 nt)	Female	normal	Enlarged and deformed
n5-8	−7 nt (+11 nt)	Female	rambling	Poorly developed
n5-13	−4 nt, −3 nt	Female	rambling	Enlarged and deformed
n5-24	−34 nt, +59 nt	Male	normal	Poorly developed
n5-10	−4 nt (−6 nt, +2 nt)	Male	normal	Enlarged and deformed
n5-6	−4 nt, −3 nt (−2 nt)	Male	normal	Enlarged and deformed

^#Large deletions (−) and insertions (+) were detected by gel electrophoresis of PCR fragments amplified from genomic DNA (see Methods) and Sanger sequenced. They were recognized as major PCR bands of non-wild-type size. For PCR fragments migrating close to the predicted wild-type size, local nucleotide insertions and deletions were detected by TIDE analysis (see Methods). Genotypes were classed as “predominant” if they correspond to major PCR bands from gel analysis or were estimated by TIDE analysis as more than 20% of the genotype, and as “minor” if estimated at between 5% and 20% in TIDE analysis. Any additional local mutations estimated at under 5% by TIDE analysis are not included.

*See Fig 2A.

Abbreviations: MIHR, MIH receptor; TIDE, Tracking of Indels by Decomposition