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. 2020 Mar 3;15(3):e0227094. doi: 10.1371/journal.pone.0227094

Fig 2. Longitudinal CWD prion fecal shedding in 96 GS deer.

Fig 2

(A) Representative RT-QuIC data curves from CWD positive deer, 1310, at multiple sampling timepoints and negative controls. Data from these graphs is expressed as reaction rates (1/time to cross threshold) as described previously and shown below. (B) Five collection time points encompassing fecal prion shedding are displayed for deer 1305, 1306, 1307, and 1310. Y-axis displays amyloid formation rate for CWD seeding activity at each sampling point displayed on the x-axis as months post inoculation (MPI). Below MPI, IHC and RT-QuIC data are displayed for RAMALT biopsies taken at the same collection. For IHC or RT-QuIC testing in which nt is entered, no sample was taken due to previous positive IHC results. For IHC, nf signifies that no follicles were present in the biopsy sample. For RT-QuIC feces, nc denotes that no sample was collected at the timepoint. Negative control fecal samples were also included, which were collected from CWD negative deer (deer # 123, 502, 814, and 819). Data shown is the amyloid formation rate of 8 replicates with mean ± SEM; * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.