Fig 4. CWD seeding activity is decreased by seven rounds of freezing and thawing of deer fecal pellets.

Deer fecal pellets were tested prior to freezing and thawing and then after 7 cycles of freeze/thaw. Graphs show amyloid formation rate from feces of deer at certain collection timepoints post infection. (A) Fecal samples from CWD positive deer initially tested positive but seeding activity was lost after 7 freeze/thaw cycles. (B) Fecal samples from CWD negative deer were affected by freeze/thaw as all negative for seeding activity initially and remained negative after 7 freeze/thaw cycles. Data shown are the amyloid formation rate of 8 replicates with mean ± SEM; * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.