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The effect of 6a-9 on the NL4-3 capsid assembly in vitro at 3M NaCl. (A) Capsid assembly was monitored by an increase in turbidity using a spectrophotometer at 350 nm over 19 minutes. Capsid was used at a final concentration of 30 μM, and compounds 6a-9 and PF-74 at a final concentration of 50 μM. (B) Slope/velocity quantification of capsid assembly during the first 2 minutes. Experiments were performed in triplicate. (AU) Absorption unit.
