Fig. 5.
Hydrophobic BA pool in Cyp2c70ako stimulates biliary cholesterol secretion. Gallbladders of Cyp2c70ako mice and controls with or without PX (10 mg/kg/day) were cannulated and bile flow (A), biliary secretion rates of total BAs (B), phospholipids (C), and cholesterol (D) were determined. The composition of biliary BAs (E) was determined using LCMS and used to calculate the hydrophobicity index according to Heuman (19) (see text) (F) (n = 6–8 animals per group). *P < 0.05, **P < 0.01, ***P < 0.001 (Kruskal-Wallis H test followed by Conover post hoc comparisons). CTRL, control.