Fig. 7.

Translation inhibition decreases the amounts of WT and R1771C apo(a) in transfected cells. Huh7 cells were transfected with WT (A) and R1771C (B) apo(a)-GFP vectors. Cell lysates and media were harvested after 24 h of transfection (time 0) or incubated with fresh DMEM or DMEM containing 50 μg/ml of cycloheximide for either 12 or 24 h and subsequently harvested. apo(a) was immunoprecipitated from media (500 μl) as described in the Methods. Cell lysates (60 μg) and immunoprecipitated media were subjected to SDS-PAGE and Western blotting as described previously. An 18 KIV apo(a) isoform from plasma was loaded as a positive control.