Fig. 5.

Hepatocyte lipidation of SAA is dependent on ABCA1. Primary hepatocytes from C57BL/6 (C57) and ABCA1-deficient (ABCA1KO) mice were treated without or with T0901317 (5 μM) for 8 h, and then incubated in the presence or absence of 10 μg/ml mouse SAA for 18 h, as indicated. A: Cell lysates were analyzed by SDS-PAGE followed by Western blotting for ABCA1 expression; β-actin was assessed for loading control. B: Cell media were separated by NDGGE and then analyzed by Western blotting to determine the lipidation status of SAA. The results shown are representative of three independent experiments and three separate immunoblots. The migration of size standards and lipidated SAA species are indicated.