Fig. 6.

Endogenous SAA expression does not alter the lipidation of apoA-I. A, B: Primary hepatocytes were prepared from C57BL/6 mice 24 h after injection of 1 × 10¹¹ particles AdNull or AdSAA. After 8 h incubation without or with T0901317 (5 μM), cells were incubated with mouse apoA-I (10 μg/ml) for 16 h. Cell media were separated by NDGGE followed by Western blotting to determine the lipidation status of SAA (A) and apoA-I (B). The migration of size standards and lipidated apoA-I and SAA species are indicated. C: Cell lysates (20 μg protein) were analyzed by SDS-PAGE followed by Western blotting for ABCA1 expression; β-actin was assessed for loading control. D: Primary hepatocytes from C57BL/6 mice were treated with 5 μM T0901317 for 8 h and then incubated with ¹²⁵I-radiolabeled human apoA-I (5 μg/ml), apoA-II (10 μg/ml), or SAA (10 μg/ml) for 16 h. Cell media were separated by NDGGE followed by autoradiography. The migration of size standards and lipidated species are indicated.