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MPPa-PDT induces MG-63 cell cycle arrest. (A) After MPPa-PDT treatment for 12 h, the cell cycle was analyzed by flow cytometry. (B) At 12 h, whole-cell lysates were prepared to determine Cyclin D1, Cyclin E, Cyclin A and Cyclin B1 protein expression by western blotting. Data are presented as the means ± SD from three independent experiments. *P<0.05 vs. control. MPPa, pyropheophorbide-α methyl ester; PDT, photodynamic therapy; LED, cells treated with a light-emitting diode.
