Table I.
Antibody details.
| Protein name | Source | Cat. no. | Dilution | Secondary antibody |
|---|---|---|---|---|
| Cyclin D1 | CST | 2978 | 1:1,000 | Anti-rabbit |
| Cyclin E | CST | 20808 | 1:1,000 | Anti-rabbit |
| Cyclin A | CST | 4656 | 1:2,000 | Anti-mouse |
| Cyclin B1 | CST | 12231 | 1:1,000 | Anti-rabbit |
| E-cadherin | CST | 3195 | 1:1,000 | Anti-rabbit |
| MMP-2 | CST | 40994 | 1:1,000 | Anti-rabbit |
| MMP-9 | CST | 13667 | 1:1,000 | Anti-rabbit |
| Akt | CST | 4691 | 1:1,000 | Anti-rabbit |
| p-Akt | CST | 4060 | 1:2,000 | Anti-rabbit |
| mTOR | CST | 2983 | 1:1,000 | Anti-rabbit |
| p-mTOR | CST | 5536 | 1:1,000 | Anti-rabbit |
| 4EBP1 | CST | 9644 | 1:1,000 | Anti-rabbit |
| p-4EBP1 | CST | 2855 | 1:1,000 | Anti-rabbit |
| P70S6K | CST | 2708 | 1:1,000 | Anti-rabbit |
| p-P70S6K | CST | 9208 | 1:1,000 | Anti-rabbit |
| Bip | CST | 3177 | 1:1,000 | Anti-rabbit |
| IRE1α | CST | 3294 | 1:1,000 | Anti-rabbit |
| PERK | CST | 5683 | 1:1,000 | Anti-rabbit |
| Protein disulfide | CST | 3501 | 1:1,000 | Anti-rabbit |
| CHOP | CST | 2895 | 1:1,000 | Anti-mouse |
| Cleaved caspase-3 | CST | 9664 | 1:1,000 | Anti-rabbit |
| Cleaved PARP | CST | 5625 | 1:1,000 | Anti-rabbit |
| LC-3 | CST | 13118 | 1:1,000 | Anti-rabbit |
| P62 | CST | 23214 | 1:1,000 | Anti-rabbit |
| p-PERK | Santa Cruz | Sc-32577 | 1:1,000 | Anti-rabbit |
| β-actin | CST | 4970 | 1:1,000 | Anti-rabbit |
| Goat-anti-rabbit | Invitrogen | G-21040 | 1:10,000 | |
| Goat-anti-mouse | Invitrogen | 31430 | 1:10,000 |
MMP, matrix metallopeptidase; 4EBP1, eukaryotic translation initiation factor 4E-binding protein 1; IRE1α, serine/threonine-protein kinase/endoribonuclease IRE1; PERK, eukaryotic translation initiation factor 2α kinase 3; CHOP, C/EBP-homologous protein 10; PARP, poly (ADP-ribose) polymerase 1; LC-3, microtubule-associated protein 1 light chain 3α; p, phosphorylated; CST, Cell Signaling Technology, Inc.