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. 2020 Feb 5;45(4):1073–1080. doi: 10.3892/ijmm.2020.4488

Figure 2.

Figure 2

ID-SDC-1 and ED-SDC-1 location in LNCaP cells with ectopic SNAIL or SLUG expression. (A and C) Western blot analysis of SNAIL, SLUG, vimentin and E-cadherin protein levels. (B and D) Quantification of the western blot analysis data. Data were analyzed using a Student's t-test. (E) Confocal microscopy of DAPI (nuclei), RFP (transduction control) and ID-SDC-1 (green) in EV, SNAIL or SLUG-transduced cells. (F) Nuclear ID-SDC-1 quantification (integrated optical density per area, arbitrary units). Data were analyzed using ANOVA followed by a Tukey post hoc test. (G) Colocalization of ID-SDC-1 with DAPI was assessed using Manders' overlap coefficient. Data were analyzed using analysis of variance followed by a Tukey post hoc test. (H) Confocal microscopy of DAPI (nuclei), RFP (transduction control), ED-SDC-1 (green) and CD44 (blue) in EV, SNAIL or SLUG-transduced cells. Scale bar=10 µm. (I) Colocalization of ED-SDC-1 with CD44 was assessed using Manders' overlap coefficient. Data were analyzed using ANOVA followed by a Tukey post hoc test. The data represent the average of 3 independent experiments, and the data are presented as the mean ± standard error of the mean. *P<0.05, **P<0.01, ***P<0.001. SDC-1, syndecan-1; ED, extracellular domain; ID, intracellular domain; SNAIL, zinc finger protein SNAI1; SLUG, zinc finger protein SNAI2; EV, empty vector; RFP, red fluorescent protein; ANOVA, analysis of variance; CD44, CD44 antigen.