Figure 1.

Scutellarein treatment inhibited cell viability and promoted cell apoptosis in colon cancer cells (CL-40, SW480 and T84). The cells were exposed to scutellarein (0, 20, 40, 60 and 80 µM) for 24 h, and then assays were performed. (A) Cytotoxic effect of scutellarein on CL-40 cells were determined using an MTT assay, and the IC₅₀ value was determined to be 40.9 µM. (B) Effect of scutellarein treatment on the apoptosis rate of CL-40 cells was evaluated by using a flow cytometry assay. (C) Cell viability of SW480 cells was determined using an MTT assay, and the IC₅₀ value was determined to be 40 µM. (D) SW480 cell apoptosis was detected by using flow cytometry technology. (E) Cytotoxic effect of scutellarein on T84 cells was assessed using an MTT assay, and the IC₅₀ value was determined to be 39.6 µM. (F) Cell apoptosis in T84 cells was measured using a flow cytometry assay. The data were represented as the mean ± standard error of the mean (n=3). ^*P<0.05, ^**P<0.01 vs. the control group. IC₅₀, half maximal inhibitory concentration.