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. 2020 Feb 10;45(4):1059–1072. doi: 10.3892/ijmm.2020.4496

Figure 2.

Figure 2

Scutellarein treatment decreased RAGE protein levels in SW480 and T84 cells. Western blot analysis was performed to determine the effects of scutella-rein on the expression of CD44, CD50, CD74, CD138, MCAM, CD151, CD166, CD206, RAGE and Madcam1 once the cells were treated with scutellarein (40 µM) in (A) SW480 cells and (B) T84 cells, and GAPDH was used as a loading control. (C) mRNA expression levels of RAGE were determined by using a reverse transcription-quantitative PCR assay once the cells were treated with scutellarein (40 µM). (D) Western blot analysis was performed to investigate the expression of RAGE protein once cells were transfected with the OE-RAGE plasmid together with scutellarein (40 µM) or not. The data demonstrated are representative of three independent experiments. n=3. *P<0.05 vs. the control group; #P<0.05 vs. the OE-RAGE group. CD, cluster of differentiation; MCAM, melanoma cell adhesion molecule; RAGE, receptor for advanced glycation end products; Madcam1, mucosal addressin cell adhesion molecule 1; OE, overexpression.