Figure 3.

Scutellarein treatment weakened the protein stability of RAGE and increased its ubiquitination. (A) SW480 and (B) T84 cells were exposed to 40 µM scutellarein for 24 h, and then the cells were treated with CHX (100 µg/ml) for 1, 2, 4, 8 or 24 h, followed by a western blot assay to detect the expression levels of RAGE. (C) An IP assay was used to detect the effect of scutellarein (40 µM) treatment on the ubiquitination of the RAGE protein. The data presented are representative of three independent experiments. ^*P<0.05 vs. the control group. RAGE, receptor for advanced glycation end products; CHX, cycloheximide; IP, immunoprecipitation; Ub, ubiquitin.