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. 2020 Feb 10;45(4):1059–1072. doi: 10.3892/ijmm.2020.4496

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of scutellarein on the combination of CDC4 and RAGE proteins. (A) Western blot analysis was performed to investigate the effects of scutellarein (40 µM) on the expression of ubiquitin-associated proteins, including CDC4, RCHY1, MDM2, CBL and Smurf1 in SW480 cells. (B) An IP assay was performed to detect the effects of scutellarein on the combination of CDC4 and RAGE in SW480 cells. (C) Representative data presenting the levels of ubiquitin-associated proteins (CDC4, RCHY1, MDM2, CBL and Smurf1) in T84 cells treated with scutellarein. (D) An IP assay was used to assess the effects of scutellarein on the combination of CDC4 and RAGE proteins in T84 cells. n=3. ^*P<0.05 vs. the control group. RAGE, receptor for advanced glycation end products; CDC4, cell division control protein 4; IP, immunoprecipitation; RCHY1, ring finger and CHY zinc finger domain containing 1; MDM2, MDM2 proto-oncogene; CBL, Cbl proto-oncogene; Smurf1, SMAD specific ubiquitin protein ligase 1.