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. 2020 Feb 10;45(4):1059–1072. doi: 10.3892/ijmm.2020.4496

Figure 6.

Figure 6

Effects of CDC4/RAGE on the proliferation and apoptosis of SW480 and T84 cells. Following 24 h of cell transfection with OE-NC or OE-CDC4, (A) SW480 and (B) T84 cells were treated with CHX (100 µg/ml) for 1, 2, 4, 8 or 24 h, and then a western blot assay was performed to detect the protein levels of RAGE. (C) A western blot assay was performed to investigate the ubiquitination of RAGE subsequent to SW480 and T84 cells being transfected with OE-CDC4 or OE-NC. Cell proliferation of (D) SW480 and (E) T84 cells was detected using MTT analysis once the cells were transfected with OE-CDC4, OE-CDC4+OE-PAGE or their negative control vector. (F) Effects of CDC4 and RAGE overexpression on the apoptosis of SW480 and T84 cells were analyzed using flow cytometry. n=3. *P<0.05 vs. control group; #P<0.05 vs. the OE-CDC4 group. RAGE, receptor for advanced glycation end products; CDC4, cell division control protein 4; OE, overexpression; NC, negative control; CHX, cycloheximide.