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. 2020 Feb 10;45(4):1059–1072. doi: 10.3892/ijmm.2020.4496

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Scutellarein treatment decreased RAGE expression and induced cell apoptosis via increasing CDC4 expression. (A) Reverse transcription-quantitative PCR and (B) western blot assays were used to determine the knockdown efficiency of sh-CDC4 in SW480 and T84 cells. ^*P<0.05 vs. the sh-NC group. Next, SW480 and T84 cells were divided into three groups (control, scutellarein and scutellarein+sh-CDC4), and then submitted to the following assays. (C) Expression of RAGE was detected using a western blot assay. Protein stability of RAGE was detected by using the CHX reagent together with a western blot assay in (D) SW480 and (E) T84 cells. Levels of cleaved caspase3/7 and total caspase3/7 were determined using a western blot assay in (F) SW480 and (G) T84 cells. ^*P<0.05 vs. the control group; ^#P<0.05 vs. the scutellarein group. RAGE, receptor for advanced glycation end products; CDC4, cell division control protein 4; sh-, short hairpin RNA; NC, negative control; CHX, cycloheximide.

Scutellarein treatment decreased RAGE expression and induced cell apoptosis via increasing CDC4 expression. (A) Reverse transcription-quantitative PCR and (B) western blot assays were used to determine the knockdown efficiency of sh-CDC4 in SW480 and T84 cells. ^*P<0.05 vs. the sh-NC group. Next, SW480 and T84 cells were divided into three groups (control, scutellarein and scutellarein+sh-CDC4), and then submitted to the following assays. (C) Expression of RAGE was detected using a western blot assay. Protein stability of RAGE was detected by using the CHX reagent together with a western blot assay in (D) SW480 and (E) T84 cells. Levels of cleaved caspase3/7 and total caspase3/7 were determined using a western blot assay in (F) SW480 and (G) T84 cells. ^*P<0.05 vs. the control group; ^#P<0.05 vs. the scutellarein group. RAGE, receptor for advanced glycation end products; CDC4, cell division control protein 4; sh-, short hairpin RNA; NC, negative control; CHX, cycloheximide.