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. 2020 Feb 4;45(4):993–1004. doi: 10.3892/ijmm.2020.4483

Figure 3.

Figure 3

Effects of APE1 knockdown on cytotoxicity and apoptosis in the presence or absence of curcumin in SH-SY5Y cells exposed to OGD/R. SH-SY5Y cells were transfected with APE1t or APE1s, followed by treatment with curcumin (10 µl) for 24 h immediately after 1 h of OGD exposure. (A) Western blot and (B) quantified protein expression of APE1. **P<0.01 vs. control. (C) Cell viability was measured by Cell Counting Kit-8 assay. (D) LDH activity was detected with a cytotoxicity detection kit. (E) Caspase-3 activity was measured using an assay kit. (F) The protein expression levels of Bax and Bcl-2 were determined by western blot analysis. Quantitative analysis of the (G) Bax and (H) Bcl-2 protein bands. Data are expressed as the mean ± standard deviation of at least three independent experiments. *P<0.05 and **P<0.01, vs. APE1s + control; #P<0.05 and ##P<0.01, vs. APE1s + OGD/R exposure alone; &P<0.05 and &&P<0.01, vs. APE1s + OGD/R + curcumin treatment. OGD/R, oxygen-glucose deprivation/reperfusion; APE1, apurinic/apyrimidinic endonuclease 1; APE1t, siRNA targeting APE1; APE1s, scrambled siRNA; LDH, lactate dehydrogenase; Bax, Bcl-2-associated X protein; Bcl-2, B-cell lymphoma 2; NS, no significant difference.