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. 2020 Feb 13;45(4):1081–1090. doi: 10.3892/ijmm.2020.4497

Figure 1.

Figure 1

PFD prevents Hcy-induced cardiomyocyte cytotoxicity. (A) Dose-dependent cytotoxicity of Hcy towards H9c2 cells. H9c2 cells were exposed to 0-3 mmol/l Hcy for 24 h. (B) Cytotoxicity of PFD towards H9c2 cells. Cells were treated with PFD (0-1.5 mg/ml) for 24 h. (C) PFD alleviated Hcy-induced cytotoxicity in H9c2 cells. Cells were pre-treated with or without (0-1.5 mg/ml) PFD for 30 min and co-treated with 3 mM Hcy for 24 h. (D) The cellular morphology of H9C2 cardiomyocytes was examined under an inverted phase contrast microscope (magnification, ×40). Cell viability following treatment was detected by CCK-8 assay. *P<0.05, **P<0.01 vs. control; #P<0.05, ##P<0.01, ###P<0.001 vs. Hcy exposure. Data are shown as the means ± SE (n=3). Hcy, homocysteine; PFD, pirfenidone.