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(A, B and D) Fluorescence localization of the apoptotic proteins, Bax, Bcl-2 and caspase-3. Magnification, ×200. (C and E) Half of the relative fluorescence intensity of Bax/Bcl-2 and caspase-3 quantitative analysis. (F and G) Hcy-induced Bax/Bcl-2 and cleaved caspase-3 mRNA upregulation was prevented by PFD. *P<0.05 vs. control; #P<0.05, ##P<0.01 vs. Hcy treatment; &P<0.05 vs. Hcy treatment. Data are shown as the means ± SE (n=3). Hcy, homocysteine; PFD, pirfenidone.
