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. 2020 Feb 13;45(4):1081–1090. doi: 10.3892/ijmm.2020.4497

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Cx43 is involved in the Hcy-induced apoptosis of H9C2 cardiomyocytes. (A) Effects of Hcy and PFD on the expression of Cx43. (B) Statistical analysis of the expression of Cx43. (C) Fluorescence localization of the apoptosis protein Cx43. Magnification, ×200. (D) Half of the relative fluorescence intensity of Cx43 quantitative analysis. (E) The effect of PFD on Cx43 mRNA expression in H9C2 cells was determined by reverse transcription-quantitative PCR. ^*P<0.05, ^**P<0.01 vs. control; ^#P<0.05 vs. Hcy treatment; ^&P<0.05 vs. Hcy treatment. Data are shown as the means ± SE (n=3). Hcy, homocysteine; PFD, pirfenidone; Cx43, connexin 43.