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. 2019 Dec 20;15(2):1706026. doi: 10.1080/15592324.2019.1706026

Figure 1.

Figure 1.

VIP1 interacts with PP2A-C subunits in a yeast two-hybrid system.

(a) The pGBKT7 vector (TaKaRa Bio, Kusatsu, Japan) containing no insert (BD: Alone), PP2A-A2 (BD: PP2A-A2), PP2A-C3 (BD: PP2A-C3), or PP2A-C5 (BD-PP2A-C5) was co-introduced with the pGADT7-Rec vector (TaKaRa Bio) containing no insert (AD: Alone) or VIP1 (AD: VIP1 FL) into the yeast strain AH109. (B) The pGBKT7 vector containing no insert (BD: Alone), PP2A-C3 (BD: PP2A-C3), or PP2A-C5 (BD-PP2A-C5) was co-introduced with the pGADT7-Rec vector containing no insert (AD: Alone) or truncated forms of VIP1, VIP1 N (amino acids 1–186; AD: VIP1 N) and VIP1C (amino acids: 165–341; AD: VIP1 C) into the yeast strain AH109. The transformed yeast cells were cultured on the double-dropout medium (DDO: synthetic dextrose (SD) medium lacking tryptophan and leucine), the quadruple-dropout medium (QDO: SD medium lacking tryptophan, leucine, histidine, and adenine), and the triple-dropout medium supplemented with 3-amino-1,2,4-triazole (3-AT) (TDO/+3-AT: SD medium that lacks tryptophan, leucine, and histidine, and that contains 5mM 3-AT) to examine activation of the reporter genes HIS3 and ADE2. Experiments were performed four times and a representative result is presented.