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. 2020 Jan 14;15(2):1714292. doi: 10.1080/15592324.2020.1714292

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© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — Mycorrhization.

(a) Real time PCR confirmed increased mycorrhizal colonization and function. Reduced SlSUT2 expression (blue) is accompanied by increased gene expression of the fungal RiGAPDH (green) or the mycorrhization-specific phosphate transporter gene SlPT4 (red). ***p < .001; **p < .01; *p < .05. (b) Arbuscules of SlSUT2-RNAi #27 show a high number of septa (arrows). (c) Tubule diameter of arbuscule branches is reduced in SlSUT2-RNAi plant. The diameter of the finest arbuscular branches was analyzed using the confocal Zeiss software ZEN 2.0 blue edition. In case of WT plants, 15 tubules from 4 different arbuscules in 8 different plants were measured (n = 480), in case of transgenic SlSUT2-RNAi plants, 15 tubules from 4 different arbuscules in 3 different plants per transgenic line were measured (n = 180). The tubule diameter of finest arbuscular branches was significantly reduced in two out of four SlSUT2-RNAi plants (*p < .05).