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. 2020 Mar 3;9:e52420. doi: 10.7554/eLife.52420

Figure 4. Heterogeneous responses of individual spines to visual deprivation.

(A) Heat map of change in sGluA1 level within individual dendrites in VD and sham-surgery (SH) groups. (B) Histogram of spine sGluA1 changes in dendrites 7 days after VD or sham-surgery (n = 23 dendrites in sham group; n = 49 dendrites in VD group; Kolmogorov-Smirnov test, p=0.079). (C) Correlation between spine sGluA1 changes of individual dendrites at day 1 and day 7 after VD (n = 49 dendrites; Pearson). (D) Changes of spine sGluA1 expression in dendrites that show decrease or no decrease at day 1 following VD in V1 L2/3 neurons. (n = 25 decrease dendrites and n = 24 no decrease dendrites from eight mice; one-way ANOVA). (E) Percentage of dendrites that decrease spine sGluA1 after 1 day of VD (51.02%). (F) Changes in spine sGluA1 of individual dendritic segments from the same V1 L2/3 neuron following binocular enucleation. Each line indicates individual dendritic segment. Data are presented as mean ± SEM. ****p<0.0001.

Figure 4.

Figure 4—figure supplement 1. Heterogeneous responses of individual spines to visual deprivation.

Figure 4—figure supplement 1.

(A) Heat map of change in sGluA1 level within individual spines in L2/3 neurons following visual deprivation. (B) Histogram of spine sGluA1 changes in mice with or without 7 days of visual deprivation (n = 272 spines in sham group; n = 690 spines in VD group; Kolmogorov-Smirnov test, p=0.0007). (C) Changes of spine sGluA1 expression in spines that show decrease or no decrease at day 1 following VD in V1 L2/3 neurons. (n = 64 decrease spines and n = 584 no decrease spines from eight mice; one-way ANOVA). **p<0.01; ****p<0.0001.