Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Biological sample | Stenopus hispidus | Salty Bottom Reef Co. | SBRC-I-CBS | n = 25 |
| Biological sample | Uca minax | Salty Bottom Reef Co. | N/A | n = 12 |
| Biological sample | Coenobita clypeatus | Carolina Biological Supply Co. | 142415 | n = 13 |
| Biological sample | Orconectes immunis | Carolina Biological Supply Co. | N/A | n = 8 |
| Biological sample | Procambarus clarkii | Carolina Biological Supply Co. | 142512 | n = 10 |
| Biological sample | Periplaneta americana | Carolina Biological Supply Co. | 143642 | n = 20 |
| Biological sample | Alpheus bellulus | LiveAquaria | CN-90061 | n = 20 |
| Biological sample | Stenopus hispidus | LIveAquaria | CN-78439 | n = 3 |
| Biological sample | Penaeus vannamei | Gulf Specimen Marine Laboratories | N/A | n = 24 |
| Biological sample | Neogonodactylus oerstedii | Gulf Specimen Marine Laboratories | Ar-1600 | n = 25 |
| Biological sample | Lebbeus groenlandicus | Friday Harbor Laboratories | N/A | n = 37 |
| Biological sample | Munida quadrispina | Friday Harbor Laboratories | N/A | n = 2 |
| Biological sample | Crangon franciscorum | Friday Harbor Laboratories | N/A | n = 10 |
| Biological sample | Spirontocaris lamellicornis | Friday Harbor Laboratories | N/A | n = 3 |
| Biological sample | Paracrangon echinata | Friday Harbor Laboratories | N/A | n = 8 |
| Biological sample | Neotrypaea californiensis | Friday Harbor Laboratories | N/A | n = 11 |
| Biological sample | Betaeus harrimani | Friday Harbor Laboratories | N/A | n = 3 |
| Biological sample | Hemigrapsus nudus | Friday Harbor Laboratories | N/A | n = 40 |
| Biological sample | Nebalia pugettensis | Friday Harbor Laboratories | N/A | n = 4 |
| Biological sample | Ligia pallasii | Friday Harbor Laboratories | N/A | n = 3 |
| Biological sample | Pagurus hirsutiusculus | Friday Harbor Laboratories | N/A | n = 35 |
| Antibody | α-Tubulin (Mouse, monoclonal) | Developmental Studies Hybridoma Bank (DHSB) | CAT#: 12G10; RRID: AB_1157911 | 1:100 |
| Antibody | α-Tubulin (Rabbit, polyclonal) | Abcam | CAT#: ab15246; RRID: AB_301787 | 1:250 |
| Antibody | Synapsin (SYNORF1; Mouse, monoclonal) | Developmental Studies Hybridoma Bank (DHSB) | CAT#: 3C11; RRID: AB_528479 | 1:100 |
| Antibody | Serotonin (5HT; Rabbit, polyclonal) | ImmunoStar | CAT#: 20080; RRID: AB_572263 | 1:1000 |
| Antibody | Glutamic acid decarboxylase (GAD; Rabbit, polyclonal) | Sigma-Aldrich | CAT#: G5163; RRID: AB_477019 | 1:500 |
| Antibody | Tyrosine hydroxylase (TH; Mouse, monoclonal) | ImmunoStar | CAT#: 22941; RRID: AB_572268 | 1:250 |
| Antibody | DC0 (Rabbit, polyclonal) | Generous gift from Dr. Daniel Kalderon (Skoulakis et al., 1993) | RRID: AB_2314293 | 1:400 |
| Antibody | RII (Rabbit, polyclonal) | Generous gift from Dr. Daniel Kalderon (Li et al., 1999) | N/A | 1:400 |
| Antibody | AffiniPure Donkey Anti-Mouse IgG (H+L) Cy3 (polyclonal) |
Jackson ImmunoResearch | CAT#: 715-165-150; RRID: AB_2340813 | 1:400 |
| Antibody | AffiniPure Donkey Anti-Rabbit IgG (H+L) Cy5 (polyclonal) |
Jackson ImmunoResearch | CAT#: 711-175-152; RRID: AB_2340607 | 1:400 |
| Antibody | AffiniPure Donkey Anti-Rabbit IgG (H+L) Alexa Flour 647 (polyclonal) | Jackson ImmunoResearch | CAT#: 711-605-152; RRID: AB_2492288 | 1:400 |
| Other (serum) | Normal donkey serum | Jackson ImmunoResearch | RRID: AB_2337258 | N/A |
| Other (DNA stain) | SYTO 13 Green Fluorescent Nucleic Acid Stain | Thermo Fisher Scientific | CAT#: S7575 | 1:2000 |
| Other (Phalloidin stain) | Alexa Fluor 488 Phalloidin | Thermo Fisher Scientific | CAT#: 12379; RRID: AB_2315147 | 1:40 |
| Other (Chemical) | α-terpineol | Sigma-Aldrich | CAT#: 432628 | N/A |
| Other (histology chemical) | Protargol (silver proteinate) | Winthrop Chemical Co. | Product discontinued | N/A |
| Other (histology chemical) | Silver proteinate | Generated in-house Followed recipe described in Pan et al. (2013) |
N/A | |
| Other (histology chemical) | Potassium dichromate | Sigma-Aldrich | CAT#: 207802 | N/A |
| Other (HPLC purified water) | HPLC Water | Sigma-Aldrich | CAT#: 270733 | N/A |
| Other (histology chemical) | Osmium tetroxide | Electron Microscopy Sciences | CAT#: 19150 | N/A |
| Other (histology chemical) | Siler nitrate | Electron Microscopy Sciences | CAT#: 21050 | N/A |
| Other (histology chemical) | Propylene oxide | Electron Microscopy Sciences | CAT#: 20401 | N/A |
| Other (embedding resin) | Durcupan ACM resin (4-part component kit) | Sigma-Aldrich | CAT#: 44610 | N/A |
| Other (mounting medium) | Permount mounting medium | Fisher Scientific | CAT#: SP15-100 | N/A |
| Software, algorithm | Photoshop CC | Adobe Inc | N/A | N/A |
| Software, algorithm | Fiji | Open-source software Schindelin et al. (2012) | z-project plugin | |
| Software, algorithm | Helicon Focus | Helicon Soft | N/A | N/A |
| Other (microscope) | LSM 5 Pascal confocal microscope | Zeiss | N/A | N/A |
| Other (microscope) | Orthoplan light microscope | Leitz | N/A | N/A |