. 2020 Jan 23;214(3):669–690. doi: 10.1534/genetics.119.302860

Table 3. LIN-29-induced seam cell fusion in earlier developmental stages.

Time of heat shock	Time observed	Strain	% Multiple or all seam cells fused	% Only one fusion between two cells	N
Late L2	Early L3	hs::control; heIs63	0	7	42
Late L2	Early L3	hs::lin-29; heIs63	45	0	42
Early L3	Mid L3	hs::control; heIs63	0	0	30
Early L3	Mid L3	hs::lin-29; heIs63	82	3	60
Early L3 + mid L3	Late L3	hs::control; heIs63	0	0	18
Early L3 + mid L3	Late L3	hs::lin-29; heIs63	100	0	30

Animals expressing GFP in the nucleus and at the plasma membrane of the seam cells (from heIs63; see Materials and Methods) and carrying either hs::lin-29 or hs::control were heat-shocked as indicated and observed for precocious seam cell fusion at the time shown. Multiple and single fusion events were scored with epifluorescence microscopy. In all cases P ≤ 0.0001 (Fisher’s exact test) compared to the corresponding hs::control.