Table 7. Relative percent of fatty acid content in animals overexpressing lin-29 vs. control.
| Fatty acid (FA) | % FA in hs::lin-29 relative to hs::control | ||
|---|---|---|---|
| Run 1 | Run 2 | ||
| C14:0 | Myristic acid | 72 | 143 |
| C15:1 | 58 | 103 | |
| C15:0 | Pentadecanoic acid | 112 | 123 |
| C16:1 | Palmitoleic acid | 102 | 155 |
| C16:0 | Palmitic acid | 82 | 87 |
| C17:2 | 50 | 60 | |
| C17:1 | Heptadecanoic acid | 69 | 81 |
| C17:0 | Margaric acid | 80 | 66 |
| C18:3 | Linolenic acid | 72 | 92 |
| C18:2 | Linoleic acid | 90 | 85 |
| C18:1 | Oleic acid | 81 | 88 |
| C18:0 | Stearic acid | 79 | 76 |
| C19:1 | 147 | 108 | |
| C19:0 | Nonadecanoic acid | 58 | 60 |
| C20:5 | Eicosapentaenoic acid | 106 | 81 |
| C20:4 | Arachidonic acid | 91 | 65 |
| C20:3 | 77 | 51 | |
| C20:2 | 61 | 73 | |
| C20:0 | Eicosanoic acid | 67 | 65 |
| Total | 84 | 82 | |
Amounts of individual fatty acid in young adults were assessed by their esterification to fatty acid methyl esters (FAMEs) and quantification via gas chromatography–mass spectrometry in hs::lin-29 and hs::control animals after heat-shock treatment in the larva (see Materials and Methods). Each run included two independent biological trials. Shown is the percentage of each FAME in hs::lin-29 animals relative to the amount in hs::control animals. “Total” indicates the sum of all FAME species in hs::lin-29 animals relative to hs::control animals.