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. 2019 Dec 30;214(3):703–717. doi: 10.1534/genetics.119.302824

Figure 2.

Figure 2

Microscope imaging of conjugation tube formation and hyphae initiation during C. neoformans sexual reproduction. (A–D) From left to right, DIC, GFP, mCherry, and merged images are shown. (A) Images of the parental strain SSH116 after incubation for 12 hr on MS solid medium. (B) Images of the parental strain SSG269 after incubation for 12 hr on MS solid medium. (C) Images of conjugation tube formation between strains SSH116 and SSG269 after 12 hr of coculturing on MS solid medium, in which the conjugation tubes are formed by the MATα strain that has the mCherry signal. (D) Images of hyphae initiation in crosses between strains SSH116 and SSG269 after 24 hr of coculturing on MS solid medium, in which the hyphae are formed from the MATa cells and on the opposite side of the conjugation tube. White arrowheads highlight the gaps between mitochondrial complexes from the zygote and the conjugation tubes. Bars, 10 μm.