Figure 3.

Effects of the CRG1 gene on mating and mito-UPI. (A) Wild-type (I) as well as unilateral (II and III) and bilateral (IV) confrontation assays of crg1Δ deletion strains. Bars, 20 μm. (B) Genotyping of the mating (STE20) and the mitochondrial types (COX1) of 40 random spores dissected from crg1Δ unilateral (I and II) and bilateral (III) crosses. The progeny highlighted with red stars are those that inherited mitochondria from the MATα parent. (C) Summary of spore dissection, germination, and mito-UPI frequencies from crg1Δ unilateral and bilateral crosses.