Table 3. Summary of genotyping results for C. neoformans mating/fusion products.
| NAD2 (mito)a | NAD5 (mito)a | STE20- Aαb | STE20- Dαb | SXI2- Aab | SXI1- Dαb | Number of fusion products (%) | |
|---|---|---|---|---|---|---|---|
| CHY1517 × YPH716 | |||||||
| CHY1517 | D | D | − | + | − | + | / |
| YPH716 | A | A | + | − | + | − | / |
| Heterozygote | H | H | + | + | + | + | / |
| Progeny genotype 1 | D | D | + | + | + | + | 80 (65) |
| Progeny genotype 2 | A | A | + | + | + | + | 37 (30) |
| Progeny genotype 3 | D | A | + | + | + | + | 6 (5) |
| JEC20 × M001 | |||||||
| JOHE50 | D | D | / | ||||
| M001 | A | A | / | ||||
| Heterozygote | H | H | / | ||||
| Progeny genotype 1 | D | D | 45 (92) | ||||
| Progeny genotype 2 | D | A | 4 (8) | ||||
| JEC20 × M049 | |||||||
| JOHE50 | D | D | / | ||||
| M049 | A | A | / | ||||
| Heterozygote | H | H | / | ||||
| Progeny genotype 1 | D | D | 41 (82) | ||||
| Progeny genotype 2 | A | A | 3 (6) | ||||
| Progeny genotype 3 | D | A | 4 (8) | ||||
| Progeny genotype 4 | D | H | 2 (4) |
a
A indicates serotype A mitochondrial type, D indicates serotype D mitochondrial type, and H indicates heterozygous mitochondrial type.
b
“+” indicates the presence of the corresponding PCR product and “−” indicates the absence of the corresponding PCR product.